The aim of this study was to develop topical spray formulation for wound healing, which would form clear transparent film at the site of application. Squalene is polyunsaturated triterpene. Squalene has many activities such as anti-oxidant, emollient, anti-cancer and skin hydration. Spray formulations contain Eudragit-L-100 as film forming polymer, polyethylene glycol-6000 as plasticizer for good plasticity of film, ethanol as a solvent and iso-propyl alcohol used for dissolving squalene. Different concentrations of squalene were added in formulation to improve wound healing activity. Formulations were filled in plastic container fitted with spray valve. Formulations were evaluated for different parameters such as delivery rate, net weight, leakage rate, spray pattern, etc. An in-vivo excision wound model on rat showed that wound excision was completely healed by 0.3% squalene spray formulation in 18 days. But 0.2% squalene and 0.3% squalene spray formulations doesn’t show significant difference for wound healing activity.
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